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C8532

Sigma-Aldrich

Sodium citrate tribasic dihydrate

for molecular biology, ≥99%

Synonym(s):

Citric acid trisodium salt dihydrate, Trisodium citrate dihydrate

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About This Item

Linear Formula:
HOC(COONa)(CH2COONa)2 · 2H2O
CAS Number:
Molecular Weight:
294.10
Beilstein:
6104939
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

Assay

≥99%

form

powder

pH

7.5-9 (25 °C, 29.4 g/L)

mp

>300 °C (lit.)

solubility

water: soluble 100 mg/mL

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES string

O.O.[Na+].[Na+].[Na+].OC(CC([O-])=O)(CC([O-])=O)C([O-])=O

InChI

1S/C6H8O7.3Na.2H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;2*1H2/q;3*+1;;/p-3

InChI key

NLJMYIDDQXHKNR-UHFFFAOYSA-K

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General description

Sodium citrate tribasic dihydrate is a stabilizer that imparts colloidal stability to gold nanoparticles (AuNPs).

Application

Sodium citrate tribasic dihydrate has been used:
  • to perform antibody retrieval for the immunohistochemical analysis of ovine ovarian sections
  • as a component of induction medium to culture Agrobacterium strain C58C1
  • to isolate human neutrophils from whole blood

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Enrique Gómez et al.
Theriogenology, 146, 39-47 (2020-02-10)
Direct transfer (DT) of cryopreserved embryos to recipients facilitates on-farm application. We analyzed a new freezing/thawing (F/T) procedure for in vitro produced (IVP) embryos, integrating: 1) an ethylene-glycol based system; 2) a culture step without protein; and 3) a synthetic protein
Fengyu Zhang et al.
BMC cancer, 20(1), 673-673 (2020-07-20)
Glioblastoma is devastating cancer with a high frequency of occurrence and poor survival rate and it is urgent to discover novel glioblastoma-specific antigens for the therapy. Cancer-germline genes are known to be related to the formation and progression of several
Simon Lalonde et al.
Genome biology, 20(1), 133-133 (2019-07-10)
Genome-wide association studies (GWAS) have identified hundreds of loci associated with coronary artery disease (CAD) and blood pressure (BP) or hypertension. Many of these loci are not linked to traditional risk factors, nor do they include obvious candidate genes, complicating
Kwi Hye Koh et al.
The Journal of clinical investigation, 129(4), 1713-1726 (2019-02-13)
Soluble urokinase receptor (suPAR) is a circulatory molecule that activates αvβ3 integrin on podocytes, causes foot process effacement, and contributes to proteinuric kidney disease. While active integrin can be targeted by antibodies and small molecules, endogenous inhibitors haven't been discovered
Fengyu Zhang et al.
Cancer cell international, 20, 197-197 (2020-06-09)
Radiotherapy, chemotherapy, and surgery have made crucial strides in glioblastoma treatment, yet they often fail; thus, new treatment and new detection methods are needed. Aberrant expression of Nanos3 has been functionally associated with various cancers. Here, we sought to identify

Protocols

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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