114375

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

• Synonym(s): 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Empirical Formula (Hill Notation): C₂₁H₁₆Br₂O₅S
• CAS Number: 115-40-2
• Molecular Weight: 540.22
• Beilstein: 359618
• EC Number: 204-087-8
• MDL number: MFCD00011681
• UNSPSC Code: 12171500
• PubChem Substance ID: 24847168
• NACRES: NA.47

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: ≥89.0% (HPLC)
• form: powder
• composition: Dye content, 90%
• technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• visual transition interval: 5.2-6.8, yellow to blue
• mp: 240 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL
• λmax: 419 nm
• ε (extinction coefficient): ≥22000 at 418-424 nm in methanol
• suitability: suitable for indicator
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• InChI key: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.

Spectrophotometric determination of serum-albumin

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves