Recommended Products
Assay
≥85% (HPLC)
Quality Level
form
solution
mol wt
(Mr = 1106.7 (DIG-11-UTP-Li4))
packaging
pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])
manufacturer/tradename
Roche
storage temp.
−20°C
General description
Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.
Application
Digoxigenin-11-UTP has been used for labeling RNA probes in:
- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
Biochem/physiol Actions
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
Quality
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
<BIG>Chen X, et al.</BIG>
Biotechniques, 56 (2014)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
A George et al.
Connective tissue research, 33(1-3), 67-72 (1995-01-01)
Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from
Yasuko Horikoshi et al.
The Journal of clinical endocrinology and metabolism, 88(2), 914-919 (2003-02-08)
Metastin is a novel peptide that was recently isolated from human placenta as the endogenous ligand of an orphan heptahelical receptor, hOT7T175. Metastin has been shown to suppress the motility of hOT7T175-transfected melanoma cells; however, studies of the physiological function
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service