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MAB1676

Sigma-Aldrich

Anti-Talin 1 Antibody, NT, a.a. 139-433, clone TA205

clone TA205, Chemicon®, from mouse

Synonym(s):

Anti-ILWEQ, Anti-TLN, Anti-talin-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

TA205, monoclonal

species reactivity

rabbit, human

should not react with

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TLN1(7094)

Specificity

Human Talin. This antibody reacts with an N-terminal epitope in talin, between amino acids 139-433. On microinjection into whole cells MAB1676 disrupts actin stress fibers and focal contacts and inhibits cell motility.

Immunogen

Epitope: N-terminus, a.a. 139-433

Application

Anti-Talin 1 Antibody, N-terminus, a.a. 139-433, clone TA205 detects level of Talin & has been published & validated for use in IP, WB, IH.
Immunoblotting, recognizes a band at ~230kDa.

Immunoprecipitation

Immunofluorescence

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain between 2 and 8°C for up to six months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Karen P Fong et al.
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Blood clot contraction is driven by traction forces generated by the platelet cytoskeleton that are transmitted to fibrin fibers via the integrin αIIbβ3. Here we show that clot contraction is impaired by inhibitors of the platelet cytosolic protease calpain. We
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Actinobacillus actinomycetemcomitans produces a leukotoxin (Ltx) that kills leukocyte function-associated antigen-1 (LFA-1)-bearing cells from man, the Great Apes and Old World monkeys. The unique specificity of Ltx for the beta2 integrin, LFA-1, suggests it is capable of providing insight into
Haizhi Wang et al.
Biochemistry, 54(18), 2806-2816 (2015-04-25)
Alzheimer's disease (AD) is characterized by the deposition of amyloid β (Aβ), a peptide generated from proteolytic processing of its precursor, amyloid precursor protein (APP). Canonical APP proteolysis occurs via α-, β-, and γ-secretases. APP is also actively degraded by
Maria Salmela et al.
Scientific reports, 7(1), 3414-3414 (2017-06-15)
Conformational activation of integrins is generally required for ligand binding and cellular signalling. However, we have previously reported that the nonactivated conformation of α2β1 integrin can also bind to large ligands, such as human echovirus 1. In this study, we

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