ABN1386

Anti-HES-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Class B basic helix-loop-helix protein 39, bHLHb39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, hHL
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• species reactivity (predicted by homology): human (based on 100% sequence homology), monkey (based on 100% sequence homology), hamster (based on 100% sequence homology), horse (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_032261
• UniProt accession no.: P35428
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... HES1(3280) ; mouse ... Hes1(15205)

Description

General description

Transcription factor HES-1 (HES-1) also known as Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Immunogen

KLH-conjugated linear peptide corresponding to the near N-terminal of Mouse HES-1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected HES-1 in mouse hindbrain, cerebellum and kidney tissue.

This Anti-HES-1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of HES-1.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Hes-1 in 10 µg of mouse liver tissue lysate.

Target description

~30 kDa observed. Uncharacterized bands maybe observed in some lysates.

Linkage

Replaces: AB15470

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable