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Key Documents

AB742

Sigma-Aldrich

Anti-Apolipoprotein B Antibody

serum, Chemicon®

Synonym(s):

ApoB

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... APOB(338)

Specificity

Apolipoprotein B. Monospecific by IEP with neat antibody vs. 2X pooled human serum and neat pooled human plasma.

Immunogen

Purified human Apolipoprotein B.

Application

Anti-Apolipoprotein B Antibody is an antibody against Apolipoprotein B for use in IP & WB.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Western Blot

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Physical form

Defibrinated, delipidized and absorbed by solid phase chromatography as required. Goat antisera in 0.05M Tris-HCl pH 7.5, 0.5M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Liver, HepG2 cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rene C Adam et al.
Journal of lipid research, 61(9), 1271-1286 (2020-07-11)
Angiopoietin-like protein (ANGPTL)3 regulates plasma lipids by inhibiting LPL and endothelial lipase (EL). ANGPTL3 inactivation lowers LDL-C independently of the classical LDLR-mediated pathway and represents a promising therapeutic approach for individuals with homozygous familial hypercholesterolemia due to LDLR mutations. Yet
Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.
Klop, B; van de Geijn, GJ; Bovenberg, SA; van der Meulen, N; Elte, JW; Birnie, E; Njo et al.
Testing null
Ubiquitination regulates the assembly of VLDL in HepG2 cells and is the committing step of the apoB-100 ERAD pathway.
Fisher, EA; Khanna, NA; McLeod, RS
Journal of Lipid Research null
The AAA-ATPase p97 facilitates degradation of apolipoprotein B by the ubiquitin-proteasome pathway.
Fisher, EA; Lapierre, LR; Junkins, RD; McLeod, RS
Journal of Lipid Research null
Jelske N van der Veen et al.
Journal of lipid research, 58(4), 656-667 (2017-02-06)
Mice lacking phosphatidylethanolamine N-methyltransferase (PEMT) are protected from high-fat diet (HFD)-induced obesity and insulin resistance. However, these mice develop severe nonalcoholic fatty liver disease (NAFLD) when fed the HFD, which is mainly due to inadequate secretion of VLDL particles. Our

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