Skip to Content
Merck
All Photos(3)

Documents

235474

Sigma-Aldrich

Acridine Orange base

Dye content 75 %

Synonym(s):

3,6-Bis(dimethylamino)acridine, Solvent Orange 15

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C17H19N3
CAS Number:
Molecular Weight:
265.35
Colour Index Number:
46005
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 75%

mp

165 °C (dec.) (lit.)

solubility

1 M HCl: 1 mg/mL, clear

λmax

488 nm

ε (extinction coefficient)

≥14000 at 227-233 nm
≥20000 at 289-295 nm
≥35000 at 486-492 nm
≥40000 at 266-272 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.

Application

Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Demethylation of Acridine Orange by Arthrobacter globiformis
Itoh K
Bulletin of Environmental Contamination and Toxicology, 60, 781-785 (1998)
Ravichandran Rekha et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 51, 191-203 (2018-11-24)
Herein, we reported a method to synthesize selenium nanowires (Cr-SeNWs) relying to purified cysteine-rich antimicrobial peptide crustin in presence of ascorbic acid. Cr-SeNWs were characterized by UV-vis, XRD, FTIR and Raman spectroscopy, as well as SEM, HR-TEM and EDAX. The
Viswanathan Vinotha et al.
Journal of photochemistry and photobiology. B, Biology, 197, 111541-111541 (2019-07-05)
Here, we report the novel fabrication of ZnO nanoparticles using the Costus igneus leaf extract. Gas chromatography-mass spectrometry (GC-MS) and proton nuclear magnetic resonance (1H NMR) spectroscopy to determine the bioactive components present in the plant extract. The synthesis of
Hui-Chuan Yu et al.
PloS one, 8(2), e55705-e55705 (2013-02-06)
Previously, we reported that cancerous inhibitor of protein phosphatase 2A (CIP2A) mediates the apoptotic effect of bortezomib in hepatocellular carcinoma (HCC). Here, we report a proteasome-independent mechanism by which bortezomib induces autophagy in HCC. Our data indicate that bortezomib activated
Samy A F Morad et al.
Cancer chemotherapy and pharmacology, 71(3), 635-645 (2012-12-25)
Acid ceramidase (AC) occupies an important place in the control of cancer cell proliferation. We tested the influence of AC inhibition on the effects of PSC 833, a P-glycoprotein antagonist with potent ceramide-generating capacity, to determine whether AC could be

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service