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General description
Sigma′s GenElute™ Soil DNA Isolation Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
- Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae
- Process all types of soil, including common soil samples and difficult samples with high humic acid
- Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution
- Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications
- Easy- to-use beads for extraction - fast without any time consuming sample grinding
Application
- Quantitative, real-time PCR
- Conventional PCR
- Southern Blot Analysis
Features and Benefits
- Process all types of soil - All types of soil samples can be processed with this kit, including common soil samples such as clay, loam and sandy soil as well as difficult soil samples with high humic acid content such as compost and manure.
- Remove all humic acid from DNA samples - The kit removes all traces of humic acid using the provided Humic Acid Removal Columns and the OSR Solution
- Rapid detection of microorganisms in soil samples - Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae.
- Fast and easy processing - Rapid spin column format allows for the isolation of DNA in under 30 minutes.
- Isolate high quality total DNA - The purified DNA is free from all inhibitors including humic acid, and ca be used directly in downstream applications including PCR.
Unit Definition
Preps
Other Notes
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
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Storage Class
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
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