SRP5348
GST protein, tag-free
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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About This Item
Productos recomendados
recombinante
expressed in E. coli
Ensayo
≥70% (SDS-PAGE)
Formulario
buffered aqueous glycerol solution
mol peso
~25 kDa
técnicas
ligand binding assay: suitable
Nº de acceso NCBI
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
Escherichia coli IAI39 ... gst> gst
gst(7152360)
Descripción general
Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.
Aplicación
GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).
Forma física
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Nota de preparación
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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