SRP0408
Histone Octamer full length human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Sinónimos:
Histone Octamer
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Ensayo
≥90% (SDS-PAGE)
Formulario
aqueous solution
mol peso
113.8 kDa
envase
pkg of 100 μg
técnicas
cell based assay: suitable
solubilidad
water: soluble
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... HIST1H2BG(8339) , HIST2H2AC(8338) , HIST3H3(8290) , HIST4H4(121504)
Descripción general
Human recombinant histone octamer consisting of 2 molecules each of histones H2A (GenBank Accession No. NM_033445) amino acids 2-130(end) with a N-terminal His-tag, H2B (GenBank Accession No. NM_003528) amino acids 2-126(end) with a N-terminal His-tag, H3 (GenBank Accession No. NM_003532) amino acids 2-137(end) with a N-terminal His-tag, and H4 (GenBank Accession No. NM_003548) amino acids 2-103(end) with a N-terminal His-tag, expressed in an E. coli expression system.
Research Area: Cell Signaling
The histone octamer is a versatile protein assembly that has evolved to serve two opposing functions within the cell. It is required to bind and bend DNA to achieve fivefold compaction and partial charge neutralization of DNA, while also needing to release specific segments of DNA in a coordinated manner to allow the access of DNA-processing enzymes at the appropriate time. A modular assembly of histone dimers (consisting of either H2A and H2B or H3 and H4) binds to approximately 30 bp of DNA and is connected in a flexible yet stable manner to form a fundamental superhelical ′ramp′ with evenly spaced DNA-binding platforms.
The histone octamer is a versatile protein assembly that has evolved to serve two opposing functions within the cell. It is required to bind and bend DNA to achieve fivefold compaction and partial charge neutralization of DNA, while also needing to release specific segments of DNA in a coordinated manner to allow the access of DNA-processing enzymes at the appropriate time. A modular assembly of histone dimers (consisting of either H2A and H2B or H3 and H4) binds to approximately 30 bp of DNA and is connected in a flexible yet stable manner to form a fundamental superhelical ′ramp′ with evenly spaced DNA-binding platforms.
Aplicación
Histone Octamer full length human has been used to incubate with 12-mesyloxy-NVP along with recombinant human histone H4 to investigate the potential of histones as targets for covalent adduct formation by drug-derived electrophiles.
Acciones bioquímicas o fisiológicas
The nuclear DNA in eukaryotes is found to be associated with histones to form a compact complex called nucleosome. Histones neutralize the electrostatic nature of DNA and function as scaffolding proteins. Each core nucleosome contains two copies each of the core histones H2A, H2B, H3, and H4 to form an octameric complex. This octameric complex contains a central (H3-H4)2 tetramer flanked on both sides with H2A-H2B dimers. The octamer complex function in various stages of chromosome function, chromatin assembly and nucleosome formation. The histone dimer-tetramer interactions are also important in RNA transcription.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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