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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

Sinónimos:

Protease Inhibitor Solution, protease inhibitor

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About This Item

Número CE:
200-664-3
Número MDL:
MFCD00677817
Código UNSPSC:
12352200
NACRES:
NA.77
Formulario:
DMSO solution
solubilidad:
water: soluble
temp. de almacenamiento:
−20°C

Nombre del producto

Cóctel de inhibidores de proteasas, for use in tissue culture media, DMSO solution

Nivel de calidad

200

Formulario

DMSO solution

solubilidad

water: soluble

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Categorías relacionadas

Descripción general

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Especificidad

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Aplicación

Protease Inhibitor Cocktail has been used:
  • as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
  • to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
  • in trypsin solution for trypsin-inhibited studies
  • as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Acciones bioquímicas o fisiológicas

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Características y beneficios

  • Broad specificity
  • Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
  • Contains no metal chelators: Ensures compatibility with downstream applications
  • Convenient packaging: 1 mL in glass bottle
  • Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Componentes

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Precaución

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Cantidad

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Forma física

Solution in DMSO (D 2650, Hybri-Max).

Otras notas

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000327277
0000182627
0000198327
019M4189V
029M4044V

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Contenido relacionado

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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