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N3641

Sigma-Aldrich

4-Nitrophenyl α-L-arabinofuranoside

chromogenic, ≥98% (TLC), powder

Sinónimos:

4-nitrophenyl-ARA

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About This Item

Fórmula empírica (notación de Hill):
C11H13NO7
Número de CAS:
6892-58-6
Peso molecular:
271.22
Beilstein/REAXYS Number:
1688357
MDL number:
MFCD00057269
UNSPSC Code:
12352204
PubChem Substance ID:
329818639
NACRES:
NA.32

product name

4-Nitrophenyl α-L-arabinofuranoside, ≥98% (TLC)

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

methanol: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@@H]1O[C@@H](Oc2ccc(cc2)[N+]([O-])=O)[C@H](O)[C@H]1O

InChI

1S/C11H13NO7/c13-5-8-9(14)10(15)11(19-8)18-7-3-1-6(2-4-7)12(16)17/h1-4,8-11,13-15H,5H2/t8-,9-,10+,11+/m0/s1

InChI key

DUYYBTBDYZXISX-UKKRHICBSA-N

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Application

4-Nitrophenyl α-L-arabinofuranoside has been used:
  • as a substrate for α-arabinofuranosidase in an enzymatic assay to determine glycosidase activities in Oenococcus oeni strains
  • to determine the enzymatic activity of α-arabinofuranosidase
  • as a p-nitrophenol linked substrate to determine its activity in Aspergillus niger NW249 culture filtrate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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José Manuel Inácio et al.
Journal of bacteriology, 190(12), 4272-4280 (2008-04-15)
The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-alpha-1,5-l-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies
Liudmila V Kozlova et al.
Planta, 241(5), 1159-1172 (2015-01-23)
Specific α- l -arabinofuranosidases are involved in the realisation of elongation growth process in cells with type II cell walls. Elongation growth in a plant cell is largely based on modification of the cell wall. In type II cell walls
Joost van den Brink et al.
Biotechnology journal, 9(10), 1329-1338 (2014-08-15)
Plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. However, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds
B C Saha et al.
Applied and environmental microbiology, 64(1), 216-220 (1998-01-22)
A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produced alpha-L-arabinofuranosidase (alpha-L-AFase) when grown in liquid culture on oat spelt xylan. An extracellular alpha-L-AFase was purified 215-fold to homogeneity from the culture supernatant by ammonium sulfate treatment, DEAE Bio-Gel A agarose
T Selwood et al.
The Biochemical journal, 254(3), 899-901 (1988-09-15)
1. Michaelis-Menten parameters for the hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside were measured as a function of pL (pH or pD) in both 1H2O and 2H2O. 2. The variation of both Vmax. and Vmax./Km with pL is sigmoid, the pK governing Vmax.
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