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N3129

Sigma-Aldrich

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

Sinónimos:

p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP

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About This Item

Fórmula lineal:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
Número de CAS:
52483-84-8
Peso molecular:
417.44
Beilstein/REAXYS Number:
6553276
EC Number:
257-949-0
MDL number:
MFCD00013173
UNSPSC Code:
12352204
PubChem Substance ID:
24897623
NACRES:
NA.83

Nombre del producto

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate

Quality Level

300

assay

≥97% anhydrous basis

form

powder

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2

InChI key

KXNNUYCXODNFBD-UHFFFAOYSA-N

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Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000381153
0000379991
0000310907
0000304195
0000381153

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Bauerle WL, et al.
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Bauerle WL, et al.
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Ziad Dahabreh et al.
World journal of stem cells, 6(4), 497-504 (2014-09-27)
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Bjarni Ásgeirsson et al.
Biochemistry and biophysics reports, 24, 100830-100830 (2020-10-27)
Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Cyclohexylamine inhibited four different APs. Co-crystallization with the cold-active Vibrio AP (VAP) was performed

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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