H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• Sinónimos: HMGCoA Reductase, HMGR
• Comisión internacional de enzimas: 1.1.1.34 (BRENDA, IUBMB)
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• Ensayo: ≥90% (SDS-PAGE)
• Nivel de calidad: 200
• Formulario: solution
• actividad específica: 2-8 units/mg protein
• mol peso: ~76 kDa (for the fusion protein)
• Nº de acceso UniProt: P04035
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... HMGCR(3156)

Descripción

Aplicación

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
• as an antigen to measure anti-HMGCR antibodies by blot analysis
• in HMG-CoA reductase activity assays
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Acciones bioquímicas o fisiológicas

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Propiedades físicas

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Definición de unidad

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

Forma física

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable