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H2126

Sigma-Aldrich

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Sinónimos:

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

Número de CAS:
37326-33-3
Comisión internacional de enzimas:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351
UNSPSC Code:
12352204
NACRES:
NA.54

conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

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General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry
  • in the isolation of putative spermatogonial stem cells (SSCs)
  • to study the effects of magnetic stimulation in magnetic hydrogel properties
  • in immunohistochemistry to visualize collagen type II distribution
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
  • for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physical form

Lyophilized powder containing lactose

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000355512
0000355503
0000296727
0000355512
0000355503

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Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface
Silva ED, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 14(7), 2375-2385 (2018)
Side Effects of Drugs Annual: A world-wide yearly survey of new data and trends in adverse drug reactions., 603-619 (2010)
Suppression of Sertoli cell tumour development during the first wave of spermatogenesis in inhibin $\alpha$-deficient mice
Haverfield JT, et al.
Reproduction, Fertility, and Development, 29(3), 609-620 (2017)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
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What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Ver todo

Protocolos

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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