DUO82040

Duolink^® In Situ Mounting Medium with DAPI

• Sinónimos: in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• Línea del producto: Duolink^®
• Nivel de calidad: 200
• técnicas: proximity ligation assay: suitable
• fluorescencia: λ_ex 360 nm; λ_em 460 nm (Zeiss Filter set 49)
• idoneidad: suitable for fluorescence
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Duolink^® proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Use the Duolink^® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
Duolink^® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink^® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy^®2 is not recommended.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

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Duolink^® In Situ mounting medium with DAPI has been used to mount coverslips in proximity ligation assay (PLA) for nuclear staining.

Características y beneficios

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Información legal

Cy is a registered trademark of Cytiva

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Warning
• Frases de peligro: H290,H412
• Consejos de prudencia: P234 - P273 - P390 - P501
• Clasificaciones de peligro: Aquatic Chronic 3 - Met. Corr. 1
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable