DUO82040
Duolink® In Situ Mounting Medium with DAPI
Sinónimos:
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
About This Item
Productos recomendados
Línea del producto
Duolink®
Nivel de calidad
técnicas
proximity ligation assay: suitable
fluorescencia
λex 360 nm; λem 460 nm (Zeiss Filter set 49)
idoneidad
suitable for fluorescence
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Categorías relacionadas
Aplicación
Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Duolink® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy®2 is not recommended.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
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Características y beneficios
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Información legal
Producto relacionado
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Met. Corr. 1
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Protocolos
Duolink® PLA Multicolor Detection Protocol
This page details the Duolink® In Situ Short Protocol for fluorescence detection
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
Contenido relacionado
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Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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