Saltar al contenido
Merck

D9692

Sigma-Aldrich

Plant Protoplast Digest/Wash Solution

Rapid isolation of viable protoplasts from plant tissue

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
41105500
NACRES:
NA.56

Quality Level

form

liquid

storage temp.

2-8°C

General description

The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.

Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Anna Kärkönen et al.
Methods in molecular biology (Clifton, N.J.), 715, 1-20 (2011-01-12)
Plant tissue cultures are an efficient system to study cell wall biosynthesis in living cells in vivo. Tissue cultures also provide cells and culture medium where enzymes and cell wall polymers can easily be separated for further studies. Tissue cultures
Akira Inoue et al.
Marine biotechnology (New York, N.Y.), 13(2), 256-263 (2010-04-16)
Functional recombinant abalone alginate lyase (rHdAly) and β-1,4-endoglucanase (rHdEG66) were expressed as secreted proteins with baculoviral expression systems. The specific activity of each recombinant enzyme, 2,490 and 18.2 U/mg for rHdAly and rHdEG66, respectively, was comparable to its native form
The cell wall.
Carpita, N., and McCann, M. et al.
Biochemistry, 52-108 null
Advanced patch-clamp techniques and single-channel analysis.
White, P. J. et al
The Journal of Experimental Biology, 50, 1037-1054 (1999)
Shiu-Cheung Lung et al.
Plant cell reports, 30(4), 473-484 (2010-11-26)
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico