D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• Código UNSPSC: 41141827
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100; 200
• Formulario: liquid
• uso: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• idoneidad: suitable for electrophoresis (DNA)
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicación

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Otras notas

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Nota de preparación

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable