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C4482

Sigma-Aldrich

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Sinónimos:

Yeast cell lysis reagent

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.56

Quality Level

200

form

solution

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Features and Benefits

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Certificados de análisis (COA)

Lot/Batch Number
0000373970
0000373971
0000342070
0000290614
0000342070

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Visite la Librería de documentos

The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)

Artículos

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

Contenido relacionado

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lisis y extracción de proteínas

Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

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