MAB6020
Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4
culture supernatant, clone 11B2-1A4, Chemicon®
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
culture supernatant
tipo de anticuerpo
primary antibodies
clon
11B2-1A4, monoclonal
reactividad de especies
rat
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotipo
IgG1
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
rat ... Cyp11B1(500892)
Especificidad
Rat p450 11beta-Hydroxylase
Inmunógeno
Aa 272-285 (KNVYRELAE GRQQS-C)
Aplicación
Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.
Research Category
Metabolism
Metabolism
Research Sub Category
Enzymes & Biochemistry
Enzymes & Biochemistry
Western blot: 1:300
Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.
Optimal working dilutions must be determined by end user.
STF fixative is available from streck laboratories, www.streck.com).
Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.
Optimal working dilutions must be determined by end user.
STF fixative is available from streck laboratories, www.streck.com).
Forma física
Supernatant. Liquid in 0.1% sodium azide.
Almacenamiento y estabilidad
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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