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Key Documents

SAB4502020

Sigma-Aldrich

Anti-NOTCH2 (Cleaved-Asp1733) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Motch B, NOTC2, NOTCH2, NTC2, Neurogenic locus notch homolog protein 2 precursor

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 100 kDa
antigen 130 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

Q04721

UniProt accession no.

Q04721

application(s)

research pathology

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

proteolytically cleaved (Asp1733)

Gene Information

human ... NOTCH2(4853)

General description

Anti-NOTCH2 (Cleaved-Asp1733) antibody detects endogenous levels of fragment of activated NOTCH2 (Cleaved-Asp1733) protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Notch 2.

Immunogen Range: 1684-1733

Biochem/physiol Actions

The NOTCH2 (notch2) gene is known to be involved in the proliferation, cell cycle progression and colony formation of cancer cells. Upregulation of the gene is observed in hepatocellular carcinoma and salivary adenoid cystic carcinoma. Deletion of NOTCH2 increases the sensitivity of the cancer cells to chemotherapeutic agents such as 5-fluorouracil. NOTCH2 plays an important role in the regulation of liver development.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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You Zou et al.
Molecular medicine reports, 14(4), 3517-3524 (2016-08-31)
Notch signaling is important during the development of a variety of human tumors. Depending on the context, Notch signaling can be either oncogenic or anti‑proliferative, and therefore, its effects in cancer are unpredictable. The aim of the present study was
Wen-Rui Wu et al.
Oncology reports, 36(1), 181-188 (2016-05-26)
The Notch pathway plays an important role in both stem cell biology and cancer. Notch2 was reported to be upregulated in human hepatocellular carcinoma (HCC) tissues. However, the biological function of Notch2 in human HCC cells has not yet been
Alicja Kamińska et al.
Reproductive biology and endocrinology : RB&E, 18(1), 30-30 (2020-04-18)
Onset of spermatogenesis at puberty is critically dependent on the activity of hypothalamic-pituitary-gonadal axis and testosterone production by Leydig cells. The aim of this study was to examine whether activation of Notch receptors and expression of Notch ligands and effector
Jing Qu et al.
Molecular and cellular biochemistry, 411(1-2), 135-141 (2015-10-03)
Many studies have explored whether the Notch signaling pathway has a tumor-suppressive or an oncogenic role in various tumors; however, the role of the Notch signaling pathway in salivary adenoid cystic carcinoma (SACC) is still unknown. In this study, we

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