S9822

Anti-Sonic Hedgehog peptide (C-terminal) antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-Dsh, Anti-Hhg1, Anti-Hx, Anti-Shh
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): immunohistochemistry: 15 μg/mL; western blot: 0.1-0.2 μg/mL
• UniProt accession no.: Q62226
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Shh(20423)

Description

General description

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog Peptide (C-terminal) recognizes mouse Sonic Hedgehog C-terminal peptide. This antibody shows less than 5% cross-reactivity with rmDhh C-terminal peptide and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198).

Immunogen

purified, E. coli-derived recombinant mouse 6× histidine-tagged sonic hedgehog C-terminal peptide.

Application

Anti-Sonic Hedgehog Peptide (C-terminal) can be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For immunohistochemistry, a working concentration of 15 μg/ml is recommended.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves