Skip to Content
Merck
All Photos(1)

Documents

RAB1317

Sigma-Aldrich

Human Cathepsin D ELISA

for serum, plasma and cell culture supernatants

Synonym(s):

Cathepsin D 

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 0.049-12 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

General description

This ELISA antibody pair detects human Cathepsin D. Other species not yet determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

Cathepsin D encoded by CTSD gene, is an aspartyl protease, plays a major role in degradation of intracellular and endocytosed protein, for maintenance of tissue homeostasis and metabolism. Cathepsin D works as an endopeptidase in the acidic environment of lysosomes. Cathepsin D is part of signal transduction and a potential target for various cancer. Increased levels of Cathepsin D is associated with type 2 diabetes. Cathepsin D regulates sphingolipid metabolism and cholesterol trafficking. Genetic variations in Cathepsin D gene is a risk factor for Alzheimer′s disease and neuronal ceroid lipofuscinosis. Cathepsin D is a key marker in atherosclerosis and level of expression is increased in atherosclerotic lesions. Cathepsin D regulates the degradation of advanced glycation end products (AGEs) and impaired activity of cathepsin D results in accumulation of AGEs in photoaged skin. Increased plasma concentration of Cathepsin D causes non-alcoholic steatohepatitis (NASH) leading to hepatic lipid accumulation and inflammation.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Walenbergh SMA, et al.
Scientific Reports, 6(3), 38278-38278 (2016)
Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes
Liu L, et al.
Disease Markers, 2017 (2017)
Correlation between cathepsin D serum concentration and carotid intima-media thickness in hemodialysis patients
Moallem SA, et al.
International Urology and Nephrology, 43(3), 841-848 (2011)
Cathepsin D, a lysosomal protease, regulates ABCA1-mediated lipid efflux
Haidar B, et al.
The Journal of Biological Chemistry, 281(52), 39971-39981 (2006)
Cathepsin D contributes to the accumulation of advanced glycation end products during photoaging
Xu X, et al.
Journal of Dermatological Science, 90(3), 263-275 (2018)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service