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R0886

Sigma-Aldrich

Monoclonal Anti-Rat IgM antibody produced in mouse

clone RTM-32, ascites fluid

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

RTM-32, monoclonal

contains

15 mM sodium azide

technique(s)

capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:1,000
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal anti-Rat IgM (mouse IgG1 isotype) is derived from the RTM-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat IgM myeloma preparation. Immunoglobulin M (IgM) is the first immunoglobulin to be produced, which is detected as early as 9 weeks of gestation. It has a pentameric structure, in which monomers are linked together by disulfide bonds. IgM is produced by B cells and is expressed in high concentrations in blood.
Rat immunoglobulins have seven distinct classes, namely, IgM, IgA, IgE, IgG1, IgG2a, IgG2b and IgG2c. Rat IgMs have been implicated in complement activation . Rat IgMs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgM is a useful tool for the analysis of target proteins
Monoclonal Anti-Rat IgM antibody is specific for an epitope present on the heavy chain of rat IgM. By immunoblotting, the antibody detects the denatured-reduced heavy chain molecule of rat IgM (μ-chain). The product recognizes rat IgMs derived from normal serum or myeloma proteins, but does not detect other rat immunoglobulins. In assays such as indirect ELISA and dot blot, the antibody weakly associates with guinea pig immunoglobulins. The product does not bind to IgG or serum preparations obtained from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.

Specificity

Monoclonal anti-Rat IgM recognizes an epitope located on the heavy chain of rat IgM. The antibody detects the rat IgM derived from normal serum or myeloma proteins, but not the other rat immunoglobulins.

Immunogen

Rat IgM myeloma preparation

Application

Monoclonal Anti-Rat IgM antibody is suitable for use in:
  • indirect ELISA (1:1,000)
  • immunocytochemistry
  • immunohistochemistry
  • dot blot
  • immunoblotting

Biochem/physiol Actions

Immunoglobulin (IgM) serves as the first line of defense during microbial infections. It serves as the antigen receptor of naive B cells. Secreted IgM (sIgM) is implicated in B cell maturation and complement activation. Detection of IgM antibodies in a neonate′s serum may indicate intrauterine infection (e.g. congenital rubella syndrome). Development of anti-donor IgM after combined liver-kidney transplantation provides a graft-protecting effect. Anti-Human IgM (μ-specific) antibodies are used in the detection of IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMID) and the hyper immunoglobulin M (hyper-IgM or HIGM) syndromes.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reenu K Malhotra et al.
Archives of pathology & laboratory medicine, 132(5), 847-850 (2008-05-10)
Gastroenteropancreatic neuroendocrine tumors are uncommon tumors representing 2% of all gastrointestinal tumors. We report a case of a 21-year-old man with X-linked hyperimmunoglobulin M (hyper-IgM) syndrome who presented with diarrhea and jaundice. An ultrasound and magnetic resonance imaging showed multiple
Graham A Tipples et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 30(3), 233-238 (2004-05-12)
Rubella virus generally causes a mild fever, rash illness similar in clinical presentation to infections by other viruses including measles and parvovirus B19. Rubella infections in pregnant women in the first trimester carry a high risk of congenital rubella syndrome
Niubel Diaz Padilla et al.
Surgery, 142(5), 722-733 (2007-11-06)
The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh)
Mohan S Maddur et al.
Clinical reviews in allergy & immunology, 58(2), 213-228 (2019-06-05)
Natural antibodies (nAbs) are most commonly defined as immunoglobulins present in the absence of pathological conditions or deliberate immunizations. Occurrence of nAbs in germ- and antigen-free mice suggest that their production is driven, at least in part, by self-antigens. Accordingly
Marc F Goldstein et al.
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 97(6), 717-730 (2007-01-05)
To review and compare previously reported cases of selective IgM immunodeficiency (SIgMID) with the largest adult cohort obtained from a retrospective analysis of an allergy and immunology practice. Publications were selected from the English-only PubMed database (1966-2005) using the following

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