NB4 1A3

89121405, mouse nerve, Neuroblast

• UNSPSC Code: 41106514

Properties

• product name: NB4 1A3, 89121405
• biological source: mouse nerve
• growth mode: Adherent
• karyotype: Unstable
• morphology: Neuroblast
• products: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse C-1300 Neuroblastoma

Cell Line Description

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Application

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Culture Medium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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