B2926
Brucella Agar with Hemin and Vitamin K1
suitable for microbiology, NutriSelect® Plus
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About This Item
UNSPSC Code:
41171607
NACRES:
NA.85
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
pH
7.0±0.2 (25 °C)
application(s)
clinical testing
food and beverages
veterinary
microbiology
suitability
anaerobic bacteria
nonselective for Bacteroides spp.
nonselective for Brucella spp.
nonselective for Clostridium spp.
Application
Used for the isolation and subculture of anaerobes.
Components
Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium Bisulphite 0.10
Hemin 0.10
Vitamin K1 0.01
Agar 15.00
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium Bisulphite 0.10
Hemin 0.10
Vitamin K1 0.01
Agar 15.00
Preparation Note
Suspend 43.1 g of Brucella Agar with Hemin and Vitamin K1 in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add sterile 5% v/v defibrinated sheep blood.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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Sutter, V.L, et al.
Wadsworth Anaerobic Bacteriology Manual (1985)
A B Onderdonk et al.
Infection and immunity, 10(6), 1256-1259 (1974-12-01)
An animal model simulating intra-abdominal sepsis was produced by implanting large bowel contents into the pelvic region of rats. Bacteriological analysis of infected sites showed quantitative differences according to the stage of disease. During the initial, often lethal, peritonitis stage
W M Weinstein et al.
Infection and immunity, 10(6), 1250-1255 (1974-12-01)
An animal model has been developed to study the evolution of intra-abdominal abscesses. Gelatin capsules containing pooled colonic contents and barium sulfate were prepared in an anaerobic chamber and implanted into the pelvic region of Wistar rats. The natural course
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