MAB8754
Anti-Eastern Equine Encephalitis Antibody, Virus, clone 1A4B.6
clone 1A4B.6, Chemicon®, from mouse
Synonym(s):
EEE
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About This Item
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
1A4B.6, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
inhibition assay: suitable (hemagglutination)
isotype
IgG2b
shipped in
wet ice
Specificity
The MAb 1A4B-6 is an IgG2b antibody that reacts with an
E1 epitope shared by all alphaviruses. Encephalitis Virus: Strains Indentified:
Eastern Equine (EEE) N. American eastern equine (NJ/60);
S. American eastern equine (BeAn 5122)
Western Equine (WEE) Western equine (McMIllan); Highlands J (B-230);
Sindbis
Semliki Forest Semliki Forest
Venezuelan Equine (VEE) 1AB (TC-83 Vaccine, Trinidad Donkey, PTF-39);
1C (P676); 1D (3880); 1E ( Mena II);
1F(78V-3531); 2 (Fe3-7c); 3 (Mucambo);
4 (Pixuna); 5 (Cabassou)
E1 epitope shared by all alphaviruses. Encephalitis Virus: Strains Indentified:
Eastern Equine (EEE) N. American eastern equine (NJ/60);
S. American eastern equine (BeAn 5122)
Western Equine (WEE) Western equine (McMIllan); Highlands J (B-230);
Sindbis
Semliki Forest Semliki Forest
Venezuelan Equine (VEE) 1AB (TC-83 Vaccine, Trinidad Donkey, PTF-39);
1C (P676); 1D (3880); 1E ( Mena II);
1F(78V-3531); 2 (Fe3-7c); 3 (Mucambo);
4 (Pixuna); 5 (Cabassou)
Immunogen
Epitope: Virus
Application
Detect the Eastern Equine Encephalitis protein using this Anti-Eastern Equine Encephalitis, Virus, clone 1A4B.6 validated for use in ELISA, IF & Hemagglutination Inhibition.
ELISA
Indirect Immunofluorescence
Hemagglutination Inhibition
Optimal working dilutions must be determined by end user.
Indirect Immunofluorescence
Hemagglutination Inhibition
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% Sodium Azide.
Storage and Stability
Maintain at 2 to 8°C for up to 12 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay
The American Journal of Tropical Medicine and Hygiene, 65, 208-213 (2001)
Efficient functional pseudotyping of oncoretroviral and lentiviral vectors by Venezuelan equine encephalitis virus envelope proteins
Journal of virology, 79, 756-763 (2005)
Biotechnology journal, 16(8), e2100008-e2100008 (2021-06-28)
Western equine encephalitis virus (WEEV) causes lethal encephalitis in humans and equines, and it poses a serious public health threat in many countries. Therefore, the development of an efficient vaccine remains an important challenge for the prevention of WEEV infection.
Journal of clinical microbiology, 38(5), 1827-1831 (2000-05-02)
Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent assays (ELISAs) for the detection of anti-arboviral immunoglobulin G (IgG ELISAs) were developed for a comprehensive array of medically important arboviruses from the Alphavirus, Flavivirus, and Bunyavirus genera. Tests were optimized and standardized so
Cell, 186(12), 2672-2689 (2023-06-10)
Alphaviruses are RNA viruses that represent emerging public health threats. To identify protective antibodies, we immunized macaques with a mixture of western, eastern, and Venezuelan equine encephalitis virus-like particles (VLPs), a regimen that protects against aerosol challenge with all three
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