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IF007

Sigma-Aldrich

Interferon-α A Protein, Recombinant human

Purified Recombinant Human Interferon Alpha A (Hu-IFN-alphaA & Hu-IFN-alpha2a).

Synonym(s):

Interferon alpha-2

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

95%

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Product Source: cDNA obtained from human leukocyte mRNA expressed in E. coli.
Purified Recombinant Human Interferon Alpha A (Hu-IFN-alphaA; Hu-IFN-alpha2a). Human Gene: IFNA2

Unit Definition

Specific Activity: The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alphaA) provided by the National Institutes of Health.Unit of activity measured on bovine MDBK cells challenged with vesicular stomatitis virus (VSV).

Physical form

Frozen, in PBS containing 0.1% bovine serum albumin (BSA).

Storage and Stability

After receipt, this product should be kept at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Analysis Note

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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To understand in detail the transcriptional and functional overlap of IFN-I- and IFN-II-activated responses, we used an integrative RNAseq-ChIPseq approach in Huh7.5 cells and characterized the genome-wide role of pSTAT1, pSTAT2, IRF9 and IRF1 in time-dependent ISG expression. For the

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