AB3879
Anti-Acetyl Lysine Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
acetylation (Lys)
General description
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
Specificity
Recognizes proteins acetylated on lysine residues. This antibody recognizes multiple acetylated proteins including acetylated histones, acetylated BSA, and acetylated MBP, and exhibits no reaction with non-acetylated proteins.
Immunogen
Acetylated KLH.
Application
Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.
Immunoprecipitation:
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Epigenetics & Nuclear Function
Signaling
Epigenetics & Nuclear Function
Research Sub Category
General Post-translation Modification
Histones
General Post-translation Modification
Histones
Quality
Routinely evaluated by Western Blot on Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 50% glycerol.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Annamaria Sandomenico et al.
mAbs, 8(8), 1575-1589 (2016-10-30)
Post-translational modifications (PTMs) strongly influence the structure and function of proteins. Lysine side chain acetylation is one of the most widespread PTMs, and it plays a major role in several physiological and pathological mechanisms. Protein acetylation may be detected by
PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins.
Camolotto, SA; Racca, AC; Ridano, ME; Genti-Raimondi, S; Panzetta-Dutari, GM
Testing null
Niek G J Leus et al.
Scientific reports, 7, 45047-45047 (2017-03-28)
Chronic obstructive pulmonary disease (COPD) constitutes a major health burden. Studying underlying molecular mechanisms could lead to new therapeutic targets. Macrophages are orchestrators of COPD, by releasing pro-inflammatory cytokines. This process relies on transcription factors such as NF-κB, among others.
Terry R Medler et al.
Molecular cancer research : MCR, 14(10), 994-1008 (2016-07-01)
Stat5a is a transcription factor utilized by several cytokine/hormone receptor signaling pathways that promotes transcription of genes associated with proliferation, differentiation, and survival of cancer cells. However, there are currently no clinically approved therapies that directly target Stat5a, despite ample
Susanne Elfert et al.
PloS one, 8(4), e61867-e61867 (2013-04-25)
The members of the TCF/LEF family of DNA-binding proteins are components of diverse gene regulatory networks. As nuclear effectors of Wnt/β-catenin signaling they act as assembly platforms for multimeric transcription complexes that either repress or activate gene expression. Previously, it
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