Skip to Content
Merck
All Photos(1)

Documents

1.24568

Sigma-Aldrich

Proteinase K

(from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14

Synonym(s):

Proteinase K from Tritirachium album, Tritirachium alkaline proteinase, Endopeptidase K

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
39450-01-6
Enzyme Commission number:
3.4.21.64 (BRENDA, IUBMB)
MDL number:
MFCD00132129
UNSPSC Code:
12352204
EC Index Number:
254-457-8
NACRES:
NA.51

Quality Level

400

form

solid

mol wt

28.93 kDa

pH

6.2-6.8 (20 °C, 10 g/L in H2O)

density

1.1 g/cm3

bulk density

35 kg/m3

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Analysis Note

Appearance (color): white
Appearance (description): lyophilisate
Activity (hemoglobin; pH 7.5; 37 °C): ≥ 30.0 mAnsonU/mg
Spec. activity (calc. on protein): ≥ 40 mAnsonU/mg
DNases (Nicking activity; pBR 322; 6 h; 37 °C): not detectable
RNases (RNA; 2 h; 37°C): not detectable

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

D L Baggesen et al.
Journal of clinical microbiology, 35(1), 330-333 (1997-01-01)
Studies of pairs of Salmonella enterica serovar Enteritidis isolates from different poultry flocks showed that phage type (PT) 7 may be derived from PT 1, 4, and 6. The conversion appeared to be associated with loss of lipopolysaccharide. It is
Jan P M Langeveld et al.
Veterinary research, 52(1), 59-59 (2021-04-18)
The unconventional infectious agents of transmissible spongiform encephalopathies (TSEs) are prions. Their infectivity co-appears with PrPSc, aberrant depositions of the host's cellular prion protein (PrPC). Successive heat treatment in the presence of detergent and proteolysis by a keratinase from Bacillus
Keisuke Yoshida et al.
iScience, 24(7), 102773-102773 (2021-07-20)
As space travel becomes more accessible, it is important to understand the effects of spaceflight including microgravity, cosmic radiation, and psychological stress. However, the effect on offspring has not been well studied in mammals. Here we investigated the effect of
Julia Bartuli et al.
STAR protocols, 3(1), 101116-101116 (2022-02-05)
The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their
Takaharu Sasaki et al.
Cell reports, 28(1), 202-217 (2019-07-04)
Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg-/-Rag2-/- mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2-/- mice, which lack only acquired
View All Related Papers

Articles

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

Proteinase K in Tissue Samples

Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service