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Key Documents

07-548

Sigma-Aldrich

Anti-monomethyl-Histone H3 (Lys36) Antibody

serum, Upstate®

Synonym(s):

H3K36me1, Histone H3 (mono methyl K36)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

dot blot: suitable
immunofluorescence: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

monomethylation (Lys36)

Gene Information

human ... H3F3B(3021)

Specificity

monomethyl-Histone H3 (Lys36)

Immunogen

Peptide containing the sequence GVmeKKP corresponding to monomethyl Lysine 36 of human histone H3 .

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-monomethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in DB, IF, WB, Mplex to detect monomethyl-Histone H3 (Lys36) also known as H3K36me1, Histone H3 (monomethyl K36).

Quality

routinely evaluated by immunoblot in chicken core histones

Target description

17kDa

Physical form

0.05% sodium azide and 30% glycerol
Antiserum

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate.
Li, Yan, et al.
The Journal of Biological Chemistry, 284, 34283-34295 (2009)
Changhui Sun et al.
The Plant cell, 24(8), 3235-3247 (2012-08-16)
Chromatin modifications affect flowering time in the long-day plant Arabidopsis thaliana, but the role of histone methylation in flowering time regulation of rice (Oryza sativa), a short-day plant, remains to be elucidated. We identified a late-flowering long vegetative phase1 (lvp1)
Shuzhi Zheng et al.
Nature communications, 10(1), 1303-1303 (2019-03-23)
In plants, flowering time is controlled by environmental signals such as day-length and temperature, which regulate the floral pathway integrators, including FLOWERING LOCUS T (FT), by genetic and epigenetic mechanisms. Here, we identify an H3K27me3 demethylase, JUMONJI 13 (JMJ13), which
Han-Sae Lee et al.
The EMBO journal, 29(8), 1434-1445 (2010-03-13)
Although recent studies highlight the importance of histone modifications and ATP-dependent chromatin remodelling in DNA double-strand break (DSB) repair, how these mechanisms cooperate has remained largely unexplored. Here, we show that the SWI/SNF chromatin remodelling complex, earlier known to facilitate
Hao Yang et al.
The Plant journal : for cell and molecular biology, 85(5), 660-674 (2016-02-06)
In flowering plants, male germline fate is determined after asymmetric division of the haploid microspore. Daughter cells have distinct fates: the generative cell (GC) undergoes further mitosis to generate sperm cells (SCs), and the vegetative cell (VC) terminally differentiates. However
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