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05-581

Sigma-Aldrich

Anti-CFTR Antibody, clone MM13-4

clone MM13-4, Upstate®, from mouse

Synonym(s):

ATP-binding cassette sub-family C, member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding ca

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

MM13-4, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CFTR(1080)

General description

CFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective in ion transport. Symptoms include reduced transport of sodium chloride (saltier sweat) and digestive and respiratory disorders caused by thicker mucus. CFTR mutations are also associated with congenital bilateral absence of the vas deferens in males.

Specificity

Other species cross-reactivity not tested.
This antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates.

Immunogen

Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. Clone MM13-4.

Application

Anti-CFTR Antibody, clone MM13-4 detects level of CFTR & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.


Immunocytochemistry:
This antibody has been reported to immunostain CFTR in T84 cells.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Quality

Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or CFTR-transfected BHK.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.

Note: CFTR can run as a diffuse protein on SDS-PAGE.

Target description

170 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1k in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Pancreas. RIPA cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of CFTR folding and degradation in transiently transfected cells.
Diane E Grove,Meredith F N Rosser,Richard L Watkins,Douglas M Cyr
Methods in Molecular Biology null
Irene Cantone et al.
Nature communications, 7, 12354-12354 (2016-08-11)
Erasure of epigenetic memory is required to convert somatic cells towards pluripotency. Reactivation of the inactive X chromosome (Xi) has been used to model epigenetic reprogramming in mouse, but human studies are hampered by Xi epigenetic instability and difficulties in
Onofrio Laselva et al.
iScience, 24(6), 102542-102542 (2021-06-19)
Ivacaftor (VX-770) was the first cystic fibrosis transmembrane conductance regulator (CFTR) modulatory drug approved for the treatment of patients with cystic fibrosis. Electron cryomicroscopy (cryo-EM) studies of detergent-solubilized CFTR indicated that VX-770 bound to a site at the interface between
Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Kartner, N and Riordan, J R
Test, 292, 629-652 (1998)
Anne Bergougnoux et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 14(5), 646-653 (2015-03-24)
Although several comprehensive studies have evaluated the role of the CFTR gene in idiopathic diffuse bronchiectasis (DB), it remains controversial. We analyzed the whole coding region of the CFTR gene, its flanking regions and the promoter in 47 DB patients

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