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T7408

Sigma-Aldrich

Triethylammonium bicarbonate buffer

1.0 M, pH 8.5±0.1

Synonym(s):

Triethylammonium hydrogen carbonate buffer

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About This Item

CAS Number:
15715-58-9
Beilstein:
3624751
MDL number:
MFCD00466938
UNSPSC Code:
12161700
PubChem Substance ID:
24900453
NACRES:
NA.25

form

liquid

storage condition

(Tightly closed)

concentration

1.0 M

color

colorless

pH

8.5±0.1

density

1.02 g/mL at 25 °C (lit.)

suitability

suitable for LC-MS
suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
microbiology
sample preparation

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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Application

Triethylammonium bicarbonate buffer is used in ion-exchange chromatography and electrophoresis.
It has also been used:
  • in trypsin digestion
  • as a component of lysis buffer
  • to adjust the pH of buffer for protein quantification

Buffer for use in ion-exchange chromatography and electrophoresis.

Biochem/physiol Actions

Triethylammonium bicarbonate buffer is useful in oligonucleotides purification for electrospray mass spectrometry analysis. It is also utilized in trypsin digestion buffers.

Other Notes

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comparable product

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Methods in Molecular Biology, 109-117 (2013)
Marco L Hennrich et al.
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In proteomics, proteolytic peptides are often chemically modified to improve MS analysis, peptide identification, and/or to enable protein/peptide quantification. It is known that such chemical modifications can alter peptide fragmentation in collision induced dissociation MS/MS. Here, we investigated the fragmentation
Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
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Journal of Proteome Research, 14(2), 1308-1314 (2015)
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Hypothetical (HyP) and conserved HyP genes account for >30% of sequenced bacterial genomes. For the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough, 347 of the 3634 genes were annotated as conserved HyP (9.5%) along with 887 HyP genes (24.4%). Given the large
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