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SAB4200175

Sigma-Aldrich

Anti-Atg101 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Atg13-interacting protein, Anti-C12orf44, Anti-autophagy-related protein 101, Anti-chromosome 12 open reading frame 44

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200 μL
€448.00

€448.00


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200 μL
€448.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

€448.00


Availability
Please contact Customer Service for Availability

Request a Bulk Order

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~30 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101

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This Item
HPA039904A7356O8264
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

fractionated antiserum

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human A549 cells, rat PC12 cells, and HEK-293T cells expressing mouse Atg16l

technique(s)

western blot: 1-2 μg/mL using whole extract of human HEK-293T cells expressing ORAI1

General description

Autophagy101 (Atg101) is located on the human chromosome at 12q13.13.

Application

Anti-Atg101 antibody has been used in
  • immunoblotting
  • immunofluorescence[1]
  • immunopurification

Biochem/physiol Actions

Autophagy101 (Atg101) is a mammalian Autophagy13 (Atg13) binding protein essential for autophagy. Atg13 forms a stable complex with ULK1 (Serine/threonine-protein kinase ULK1) and FIP200 (FAK family kinase-interacting protein of 200 kDa). Atg101 associates with the ULK1-Atg13- FIP200 complex through direct interaction with Atg13. Mammalian target of rapamycin (mTOR) interacts with the ULK1-Atg13-FIP200 complex in a nutrient dependent manner, suggesting that mTOR regulates autophagy through this complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    Find documentation for the products that you have recently purchased in the Document Library.

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    Mei Yang et al.
    Science advances, 2(9), e1601167-e1601167 (2016-09-13)
    The intronic GGGGCC hexanucleotide repeat expansion in chromosome 9 open reading frame 72 (C9ORF72) is a prevalent genetic abnormality identified in both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Smith-Magenis syndrome chromosomal region candidate gene 8 (SMCR8) is a
    Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells
    Hieke N, et al.
    Autophagy, 11(9), 1471-1483 (2015)
    Systematic analysis of ATG13 domain requirements for autophagy induction
    Wallot-Hieke N, et al.
    Autophagy, 14(5), 743-763 (2018)
    Molecular Machinery and Genetics of the Autophagy Pathway
    Autophagy in health and disease, 16-16 (2012)
    Atg101, a novel mammalian autophagy protein interacting with Atg13
    Hosokawa N, et al.
    Autophagy, 5(7), 973-979 (2009)

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