Yes, a citation and user protocol for the SAB2702243 MeDIP assay can be found in the article "GGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity, BMC Genet. 2020; 21: 84.PMID : 32727353.
SAB2702243
Monoclonal Anti-5-Methylcytosine / 5-mC antibody produced in mouse
clone GT4111, affinity isolated antibody
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100 μL
€472.00
€472.00
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100 μL
€472.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
€472.00
Please contact Customer Service for Availability
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
GT4111, monoclonal
form
buffered aqueous solution
concentration
1mg/mL
technique(s)
dot blot: suitable
immunohistochemistry: suitable
methylated DNA immunoprecipitation (MeDIP): suitable
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Immunogen
The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC
Application
Suggested starting dilutions are as follows: Dot: Assay-dependent dilution, EM: Assay-dependent dilution, IHC-P: 1:100-1:1000, IHC-Wm: 1:50-1:500, MeDIP: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Other Notes
Purification: Affinity purified by Protein G
Physical form
Phosphate-buffered saline, no preservative added.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Gerhard P Aigner et al.
Toxics, 10(2) (2022-02-25)
Earthworms are well-established model organisms for testing the effects of heavy metal pollution. How DNA methylation affects cadmium (Cd) detoxification processes such as the expression of metallothionein 2 (MT2), however, is largely unknown. We therefore exposed Lumbricus terrestris to 200
Cen Jiang et al.
Cell death discovery, 8(1), 46-46 (2022-02-04)
The morbidity of papillary thyroid cancer (PTC) is on the rise, but its pathogenesis is still poorly understood. NR4A1 is a transcription factor primarily involving a wide range of pathophysiological responses, but its relationship with PTC malignancy remains unclear. This
Sunwang Xu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11(16), e2308531-e2308531 (2024-02-21)
Gallbladder cancer (GBC) is an extremely lethal malignancy with aggressive behaviors, including liver or distant metastasis; however, the underlying mechanisms driving the metastasis of GBC remain poorly understood. In this study, it is found that DNA methyltransferase DNMT3A is highly
Epigenetic activation of the elongator complex sensitizes gallbladder cancer to gemcitabine therapy.
Sunwang Xu et al.
Journal of experimental & clinical cancer research : CR, 40(1), 373-373 (2021-11-27)
Gallbladder cancer (GBC) is known for its high malignancy and multidrug resistance. Previously, we uncovered that impaired integrity and stability of the elongator complex leads to GBC chemotherapy resistance, but whether its restoration can be an efficient therapeutic strategy for
Manthan Patel et al.
BMC genetics, 21(1), 84-84 (2020-07-31)
The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. By analyzing methylated DNA-sequencing data obtained
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Is there a citation or user protocol available for the SAB2702243 MeDIP assay?
1 answer-
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Could you provide the MeDIP assay protocol or citation for SAB2702243?
1 answer-
The information you are looking for can be found at the following link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392725/
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