S4316

Amyloid Precursor Protein β, Secreted human

recombinant, expressed in E. coli (N-terminal histidine tagged), solution

• Synonym(s): Amyloid Precursor Protein β, Secreted, sAPPβ
• MDL number: MFCD06201921
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli (N-terminal histidine tagged)
• Quality Level: 200
• form: solution
• mol wt: ~100 kDa by SDS-PAGE
• UniProt accession no.: P05067
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... APP(351)

Description

General description

Amyloid-β (Aβ) peptides are a major component of the senile plaques characteristic of the Alzheimer brain.[1] It is a type- I transmembrane protein. The gene encoding amyloid-β is localized on human chromosome 21.[2][3]

Application

Human amyloid precursor protein β has been used as peptide standard.[4][5]

Biochem/physiol Actions

The secreted amyloid precursor β (sAPPβ) is a proteolytic cleavage product of β amyloid precursor protein (APP). APP is cleaved by β-secretase into two fragments, sAPPβ and β-amyloid peptide (Aβ). The Aβ is a component of amyloid plaques, one of the major hallmarks of Alzheimer`s disease (AD), while the sAPPβ is thought to modulate neuronal function and cell survival. Mutation in APP found in Swedish familial AD pedigree increases the susceptibility of APP to β-secretase cleavage and the formation of Aβ and sAPPβ. In contrast, activation of protein kinase C (PKC) decreases the levels of sAPPβ. The trans-Golgi network (TGN) is the major site for β-secretase activity.

Physical form

0.2 μm filtered solution in 20 mM Tris (pH 7.4), 0.5 M NaCl, 1 mM EDTA, 5 mM βME, 2 μg/ml aprotinin

Preparation Note

sAPPβ was expressed in E. coli as a soluble protein and purified under non-denaturing conditions

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves