R3380

Ribonucleoside vanadyl complexes

200 mM

• Synonym(s): vanadyl ribonucleoside complexes
• UNSPSC Code: 12352200
• NACRES: NA.52

Properties

• Quality Level: 200
• concentration: 200 mM
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Ribonucleoside vanadyl complexes are stable complexes formed between ribonucleotides. They are low molecular weight inhibitors of cellular ribonucleases (RNases) that are used for the isolation of RNA from cells.[1][2]

Application

Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.[3]

Ribonucleoside vanadyl complexes has been used:

• as a component of lysis buffer for cell lines prior to Poly(U) pull down assay[4]
• to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment[5]
• as a component of hybridization buffer for human retinal slices[6]

Biochem/physiol Actions

Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.[7]

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable