Prepare stock solutions in 0.1 M Tris-HCl, pH 7.0. While no specific concentration was provided, calculations based on the assay procedure suggest that stock solutions should be in the range of 0.5 - 1.0 mg/ml protein. This aligns with the general recommendation for proteins of a minimum of 1 mg protein/ml. The stock solution can be stored as aliquots at -20°C and should be reasonably stable for several months.
P4234
Pyranose Oxidase from Coriolus sp.
recombinant, expressed in E. coli, ≥2.7 units/mg solid
Synonym(s):
Pyranose: Oxygen 2-Oxidoreductase
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250 UNITS
€313.00
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250 UNITS
€313.00
1000 UNITS
€928.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
form
powder
specific activity
≥2.7 units/mg solid
shipped in
wet ice
storage temp.
−20°C
General description
Pyranose oxidase (P2O), a homotetrameric protein consists of a covalently bound flavin adenine dinucleotide (FAD). It is seen mostly among wood-degrading basidiomycetes.[1]
Application
Biochem/physiol Actions
Pyranose oxidase (P2O) can be used in clinical chemistry to determine 1,5-anhydro-d-glucitol marker, used for glycemic control in diabetes patients.[1]
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide[4].
Unit Definition
One unit produces 1.0 μmol of hydrogen peroxide per minute at 37 °C, pH 7.0.
Other Notes
Contains glutamate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Pyranose 2-oxidase (P2O) catalyzes the oxidation by O(2) of d-glucose and several aldopyranoses to yield the 2-ketoaldoses and H(2)O(2). Based on crystal structures, in one rotamer conformation, the threonine hydroxyl of Thr(169) forms H-bonds to the flavin-N5/O4 locus, whereas, in
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What is the solution stability for product P4234 after dilution?
1 answer-
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Do you have a protocol for the activity assay used to qualify pyranose oxidase?
Do you have a protocol for the activity assay used to qualify pyranose oxidase?
1 answer-
We do not have a pre-formulated protocol available at the moment. However, for reference, you may find the following publication informative. The publication, "Limited proteolysis of pyranose 2-oxidase results in a stable and active complex" in PeerJ Materials Science 2:e7 (https://doi.org/10.7717/peerj-matsci.7), served as the basis for this assay, utilizing the production of quinoneimine dye to directly reflect the turnover rate of pyranose oxidase.
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