The final buffer that this product is in prior to lyophilization is 0.1 M B-glycerophosphate and 4 mM EDTA.
A stock solution can be prepared in 40 mM glycerophosphate buffer, pH 6.8, containing 1 mM EDTA and 5 mM cysteine. EDTA is a chelating agent of copper and other metal ions, which causes oxidation of the protein. The presence of cysteine is to maintain the critical sulfhydryl group in the protein in the reduced state.
When preparing a stock solution for assaying the activity, a 0.25 unit/ml solution of the protein in 40 mM glycerophosphate buffer, pH 6.8, at 30 °C containing 80 mM cysteine is used.
P1261
Phosphorylase a from rabbit muscle
lyophilized powder, 20-30 units/mg protein
Synonym(s):
1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
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10 MG
€203.00
25 MG
€403.00
About This Item
Recommended Products
biological source
rabbit muscle
Quality Level
form
lyophilized powder
specific activity
20-30 units/mg protein
purified by
2× crystallization
composition
Protein, 30-60% E1%/280
foreign activity
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%
storage temp.
−20°C
General description
Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P). [1]
Application
Biochem/physiol Actions
Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls. [6]
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.[7]
Unit Definition
One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
Physical form
Lyophilized powder containing β-glycerophosphate and EDTA
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls inhibited both rabbit muscle phosphorylase a and phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-d-glucosyltransferase, EC 2.4.1.1). The degree of inhibition was dependent upon the relative hydrophobicity of the compounds and steric hinderance. 2,4,5,2',4',5'-Hexabromobiphenyl and Firemaster BP-6
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What is the buffer required for the enzyme activity?
1 answer-
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How is Phosphorylase A, Product P1261, prepared?
1 answer-
To prepare Phosphorylase A (P1261), Sigma uses a method similar to the one described in the J. Biol.Chem., 231, 65 (1958).
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What is the molecular weight of Phosphorylase A, Product P1261?
1 answer-
The molecular weight of Rabbit Phosphorylase A (tetramer) is 370,000 per Biochemistry 6 (1967) p. 3315 (which was determined by sedimentation and "experiments in a variety of buffers, temperatures, and ionic strength"). The molecular weight by sequence analysis (Proc. Natl. Acad. Sci. USA, 74, 4762-4766) is 97,412.
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What is the pI of Phosphorylase A, Product P1261?
1 answer-
The pI of Phosphorylase A (P1261) is 5.6 - 5.8.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Phosphorylase A, Product P1261?
1 answer-
Sigma tests solubility of Phosphorylase A (P1261) in water at a concentration of 1 mg/ml.
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Has the amino acid sequence of phosphorylase from rabbit muscle been published?
1 answer-
The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric form. The sequence information of the subunit would be the same for both forms.
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What is the final buffer Phosphorylase A, Product P1261, is in prior to lyophilization?
1 answer-
The final buffer Phosphorylase A (P1261) is in prior to lyophilization is 0.1MB-glycerophosphate and 4 mM EDTA.
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