NXTRACT
NuCLEAR™ Extraction Kit
For mammalian tissue or cultured cells
Synonym(s):
Nuclear Isolation Kit
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About This Item
UNSPSC Code:
41105517
NACRES:
NA.56
Recommended Products
Quality Level
usage
kit sufficient for 10 extractions (1 ml packed cell volume)
kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s)
protein extraction: suitable
western blot: suitable
shipped in
dry ice
storage temp.
−20°C
General description
The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
Application
NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.
Other Notes
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
Legal Information
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
Kit Components Also Available Separately
Product No.
Description
SDS
- 3× Dilution and Equilibration Buffer 90 mL
- P8340Protease Inhibitor Cocktail 1 mLSDS
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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