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MAK168

Sigma-Aldrich

Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests (Blue fluorescence)

Synonym(s):

High-Sensitivity Pyrophosphate Assay Kit

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1 KIT
€305.00

€305.00

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1 KIT
€305.00

About This Item

EC Number:
200-664-3
UNSPSC Code:
12161503
NACRES:
NA.84

€305.00

Please contact Customer Service for Availability
Request a Bulk Order

usage

sufficient for 200 fluorometric tests (Blue fluorescence)

application(s)

cosmetics
food and beverages

detection method

fluorometric

storage temp.

−20°C

General description

Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis,[1] DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.[1]

Suitability

The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.

Principle

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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Certificates of Analysis (COA)

Lot/Batch Number
3510613
3510613
3041242
3320133
2920633

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Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Tyler M Weaver et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25494-25504 (2020-10-02)
During DNA replication, replicative DNA polymerases may encounter DNA lesions, which can stall replication forks. One way to prevent replication fork stalling is through the recruitment of specialized translesion synthesis (TLS) polymerases that have evolved to incorporate nucleotides opposite DNA
Priyanka Kushwaha et al.
Journal of cellular physiology, 235(10), 6673-6683 (2020-01-28)
The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
View All Related Papers

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Any challenges encountered with decreases in fluorescence due to buffer components in the assays, such as manganese (1 mM), DTT (5 mM), and the combination of manganese with a reducing agent (both BME and DTT)? It seems that the signal is significantly reduced, if not entirely abolished, when these components are included in the buffer, even with substantial amounts of the PPi standard.

    1 answer

    1. Considering the significant quenching of fluorescence by manganese (Mn), it is not suitable for the assay. Instead, the use of MgCl2 (up to 1 mM) is suggested as it offers less interference. Additionally, DTT can be used up to 1 mM.

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