L9895
Lectin from Pseudomonas aeruginosa
lyophilized powder
Synonym(s):
PA-I
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About This Item
Recommended Products
form
lyophilized powder
Quality Level
potency
<15 μg/mL per mL agglutination activity
storage temp.
−20°C
Biochem/physiol Actions
PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.
Packaging
Package size based on protein content. Vial content based on protein by biuret.
Quality
Highly purified.
Physical form
Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride
Preparation Note
Purified by affinity chromatography using a modification of Gilboa-Garber.1
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Alessandro Palmioli et al.
Chembiochem : a European journal of chemical biology, 20(23), 2911-2915 (2019-06-20)
Biofilm formation by bacterial pathogens is a hallmark of chronic infections and is associated to increased antibiotic tolerance that makes pathogens difficult to eradicate with conventional antibiotic therapies. Infections caused by Pseudomonas aeruginosa are of great concern, especially for immunocompromised
Akiko Chiba et al.
Molecular cancer research : MCR, 18(1), 130-139 (2019-10-20)
Breast tumors have their own specific microbiota, distinct from normal mammary gland tissue. Patients with breast cancer that present with locally advanced disease often undergo neoadjuvant chemotherapy to reduce tumor size prior to surgery to allow breast conservation or limit
Articles
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