This product is a solution containing 1 nanomole or 1000 picomoles of Cytochrome P450 3A4. The concentration of several parameters varies from lot to lot and is reported in the product Certificate of Analysis. As noted in the 'PROPERTIES' section, each vial will have a range of 3.5-25 mg protein/mL, 50-150 nmol/mL P450, and 6-14 nmol/mg protein. For example, a recent lot was found to have a P450 concentration of 13 nmol/mg protein and 5.1 mg protein/mL. The volume of the 1000 picomole package for this lot is 1.5 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5042#product-documentation
H5042
Cytochrome P450 3A4 human
recombinant, expressed in E. coli
Synonym(s):
Cytochrome P450, purified, soluble, 1,4-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, CYP3A3, CYP3A4, Cytochrome P450 NF-25, Cytochrome P450-PCN1
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About This Item
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
description
purified, soluble
Assay
3.5—25 mg protein/mL (Bradford)
form
liquid
specific activity
12-200 units/mg protein
mol wt
57318.7 g/mol
concentration
3.5-25 mg protein/mL (protein)
50-150 nmol/mL (P450)
6-14 nmol/mg protein (specific content)
technique(s)
cell based assay: suitable
solubility
water: soluble
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYP3A4(1576)
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General description
Cytochrome P450 3A4 (CYP3A4) indicates a mammalian enzyme (CYP) family 3, subfamily A, gene 4. There are 57 CYP genes and 33 pseudogenes in humans, organized into 18 families and 42 subfamilies. Among these, the 3A4 isoform (CYP3A4) is the most prevalent and is primarily found in the liver and gastrointestinal tract.[1]
Application
Biochem/physiol Actions
Unit Definition
Other Notes
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Hello, Could you please provide more details about this product? Specifically, I would like to know the volume of the aliquot. Also, could you provide a more precise concentration in mg/mL?
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