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H3667

Sigma-Aldrich

Human Serum

Human Serum

Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered

Synonym(s):

HI Human Plasma, HI Human Sera, HI Human Serum, human plasma, human sera, human serum

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20 ML
€129.00
100 ML
€414.00

€129.00


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20 ML
€129.00
100 ML
€414.00

About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71
biological source:
human male plasma (Type AB)
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice

€129.00


Please contact Customer Service for Availability

biological source

human male plasma (Type AB)

Quality Level

sterility

sterile-filtered

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Serum is mostly required as a supplement to cell culture media. It contains macromolecules, carrier proteins, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors.[1]

Application

Human Serum has been used as a supplement in culture media for monocytes.[2] It has been used as a control in western blotting to study flightless I expression in serum.[3]

Packaging

Packaging may vary for different batches due to short supply of the bottles used to pack this product. The presence or lack of shrink wrap around the cap of the bottle doesn’t impact product integrity/sterility of the product.

Analysis Note

Endotoxin tested

Other Notes

It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A human and animal model-based approach to investigating the anti-inflammatory profile and potential of the 5-HT2B receptor antagonist AM1030.
Palmqvist N et al.
Journal of Inflammation (London, England), 13, 20-20 (2016)
Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression.
Kopecki Z et al.
Oncotarget, 6, 36426-36426 (2015)
Christina Megli et al.
The Journal of experimental medicine, 218(1) (2020-09-26)
The human placenta is a dynamic organ that modulates physiological adaptations to pregnancy. To define the immunological signature of the human placenta, we performed unbiased profiling of secreted immune factors from human chorionic villi isolated from placentas at mid and
Anneke H Hellinga et al.
International journal of molecular sciences, 21(23) (2020-12-03)
Bovine immunoglobulin G (bIgG) was previously shown to enhance innate immune responses to toll-like receptor (TLR) stimulation, via induction of trained immunity. In this study, we investigated whether minimally processed dairy streams with high levels of whey proteins as potential
Anubha Seth et al.
Cellular signalling, 68, 109538-109538 (2020-01-14)
Mammalian adenylate cyclases (ACs) are pseudoheterodimers with dissimilar hexahelical membrane-anchors, isoform-specifically conserved for more than half a billion years. We exchanged both membrane anchors of the AC isoform 2 by the quorum-sensing receptor from Vibrio harveyi, CqsS, which has a

Articles

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Protocols

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Questions

1–5 of 5 Questions  
  1. Is the only difference between H3667 and H4522 that H3667 is heat inactivated whilst H4522 is not? And what makes a big difference in the availability between them?

    1 answer
    1. Yes, the difference between product H3667 and H4522, is that product H3667 is heat inactivated. Any availability questions can be directed to customer service. Please navigate to the link below:https://www.sigmaaldrich.com/support/customer-support.

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  2. Is this tested for Anti HLA antibodies? I am thinking of it’s application as a flow cytometric negative control 

    1 answer
    1. This product has not been tested for the presence of anti-HLA antibodies.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    1 answer
    1. Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

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  5. Why is serum Heat-Inactivated (HI)?

    1 answer
    1. Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

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