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G9903

Sigma-Aldrich

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

F/2 Water Enrichment, Marine Enrichment Solution

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500 ML
€117.00

€117.00


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500 ML
€117.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

€117.00


Please contact Customer Service for Availability

Request a Bulk Order

sterility

sterile-filtered

Quality Level

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

General description

Guillard′s (F/2) Marine Water Enrichment Solution is composed of major nutrients, trace metals and vitamins. Guillard′s (F/2) Marine Water Enrichment Solution may be used as a chemstock to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium.

Application

Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Formula variant

With the major nutrients, trace metals and vitamins as described by Guillard (1975).

Reconstitution

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Kazuo Umemura et al.
Micron (Oxford, England : 1993), 77, 41-43 (2015-06-22)
We propose a new technique for quantitative trajectory analysis of gliding phenomenon of Navicula pavillardii (NP) and Seminavis robusta (SR) diatom cells by single cell observation using a glass microchamber in this short technical note. Two-dimensional trajectory analysis of cell
Shingo Shoumura et al.
Journal of microbiological methods, 168, 105804-105804 (2019-12-15)
Diatoms are one of the major photosynthetic planktons. Here, we studied movements of aqueous suspensions of diatoms using a home-made 'tumbled' optical microscope system. The usual inverted optical microscope was reoriented using a homemade microscope stand so that the vertical
B Marzec et al.
Journal of structural biology, 208(2), 127-136 (2019-08-23)
Coccolithophores are marine phytoplankton that are among the most prolific calcifiers widespread in Earth's oceans, playing a crucial role in the carbon cycle and in the transport of organic matter to the deep sea. These organisms produce highly complex mineralized
Yihua Xiao et al.
Journal of environmental sciences (China), 22(8), 1239-1245 (2010-12-25)
The effects of allochthonous dissolved organic matter (DOM) on the growth and photosynthesis of Phaeodactylum tricornutum were investigated. P. tricornutum incubated in f/2 medium was exposed to DOM additives, which were extracted from the plant and sediment samples of a
K H Baker et al.
Applied and environmental microbiology, 35(4), 791-796 (1978-04-01)
The marine diatom Thallasiosira pseudonanna (3H) and several bacteria associated with it were isolated from batch cultures at the University of Delaware mariculture facility. The interaction between the algae and each of the bacteria was investigated. One of the isolates

Protocols

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Questions

  1. Storage temperature is listed at -20C. For use, should it be fully thawed and refrozed every time? Is there a limit to the number of freeze/thaw cycles it will tolerate?

    1 answer
    1. This product may be thawed upon receipt and dispensed to working volumes and refrozen. Avoid repeated freeze/thaw cycles. The 1X prepared medium may be stored refrigerated for 1-2 weeks protected from light.

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