Gamborg's B-5 Basal Medium with Minimal Organics is a plant tissue culture medium that contains reduced amounts of organic compounds compared to the standard Gamborg's B-5 Basal Medium. The minimal organics version is designed to provide a lower concentration of organic compounds, such as vitamins or amino acids, which allows for more precise control over nutrient supplementation in plant tissue culture experiments. Please see the link below to review the formulation tables for Gamborg's basal salt mixtures, products G5893 and G5768.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/186/351/g5768for.pdf
G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
Gamborg’s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Synonym(s):
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
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€17.20
10 L
€88.50
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1 L
€17.20
10 L
€88.50
About This Item
UNSPSC Code:
12352207
NACRES:
NA.72
Recommended Products
form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.[1]
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa[2]
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings[3]
- as a component of solid GM medium for germination of Arabidopsis seeds[4]
Formula variant
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Quantity
Formulated to contain 3.2 grams of powder per liter of medium.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Ox. Sol. 3
Storage Class Code
5.1B - Oxidizing hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH Annex XVII (Restriction List)
CAS No.
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