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F8649

Sigma-Aldrich

Formate Dehydrogenase from Candida boidinii

lyophilized powder, 5.0-15.0 units/mg protein

Synonym(s):

FDH, Formate:NAD+ oxidoreductase

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50 UNITS
€192.00
250 UNITS
€759.00
500 UNITS
€1,120.00

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50 UNITS
€192.00
250 UNITS
€759.00
500 UNITS
€1,120.00

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

€192.00


Please contact Customer Service for Availability

Request a Bulk Order

biological source

fungus (Candida boidinii)

Quality Level

form

lyophilized powder

specific activity

5.0-15.0 units/mg protein

composition

Protein, 5.0-20.0% biuret

storage temp.

2-8°C

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Application

Formate Dehydrogenase (FDH) is used for diagnostics in large scale industrial processes. Its used in the production of an unnatural amino acid, tert-L-leucine, a component of some HIV protease and matrix metalloprotease inhibitors.[1]

Biochem/physiol Actions

Formate dehydrogenase is an abundant enzyme from yeast Candida boidinii (CbFDH) that plays an important role in the energy supply of methylotrophic microorganisms and in the stress response of plants.[1]
Formate dehydrogenase is involved in the stress response of plants [2] and catalyzes the reduction of NAD+ to NADH.[3]

Unit Definition

One unit will oxidize 1.0 μmole of formate to CO2 per min in the presence of β-NAD at pH 7.6 at 37 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Pyruvate was produced from glucose by Escherichia coli BW25113 that contained formate dehydrogenase (FDH) from Mycobacterium vaccae. In aerobic shake-flask culture (K (L) a = 4.9 min(-1)), the recombinant strain produced 6.7 g pyruvate l(-1) after 24 h with 4 g sodium formate l(-1) and a yield of
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We propose a multiple alignment of the sequence of formate dehydrogenase with the D-specific 2-hydroxy acid dehydrogenases family. Structurally conserved regions are predicted for those sequences corresponding to important regions of the catalytic and the coenzyme binding domains defined from

Questions

1–4 of 4 Questions  
  1. Could you please provide the uniprot accession code of the enzyme? Best regards

    1 answer
    1. The UniProt accession ID is O13437. Please access this link below:
      https://www.uniprot.org/uniprotkb/O13437/entry.

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  2. How do you store Formate Dehydrogenase from Candida boidinii , product F8649, once it has been solublized?

    1 answer
    1. Aliquots of the enzyme Formate Dehydrogenase from Candida boidinii , product F8649, should be stable for at least 6 months when stored at -15 to -25°C, at +33°C for 1 day, and at +2 to +8°C for 7 days. Aliquots should be thawed only once.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What solvent can Product F8649, Formate dehydrogenase from Candida boidinii be  dissolved in?

    1 answer
    1. Product F8649, Formate dehydrogenase from Candida boidinii can be dissolved in 50 mM phosphate buffer, pH 7.5 to 8.0, or in double distilled water. Solubility is approximately 10 mg/ml.

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