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D5167

Sigma-Aldrich

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 70.1, ascites fluid

Synonym(s):

Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

70.1, monoclonal

contains

15 mM sodium azide

species reactivity

marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:2,000 using a chick brain extract

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).
Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.

Immunogen

cytoplasmic dynein from chicken embryo brain

Application

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:
  • perturbation assays
  • western blotting
  • immunofluorescence microscopy
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunocytochemistry
  • immunopurification

Biochem/physiol Actions

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing
Nurminsky DI, et al.
Molecular and Cellular Biology, 18(11), 6816-6825 (1998)
Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
Casenghi M, et al.
Journal of Cell Science, 118(21), 5101-5108 (2005)
Jonne A Raaijmakers et al.
The EMBO journal, 31(21), 4179-4190 (2012-10-05)
The microtubule motor protein kinesin-5 (Eg5) provides an outward force on centrosomes, which drives bipolar spindle assembly. Acute inhibition of Eg5 blocks centrosome separation and causes mitotic arrest in human cells, making Eg5 an attractive target for anti-cancer therapy. Using
Kinesin, Dynein, and Intracellular Transport.
Lodish H, Berk A, Zipursky SL, et al
Molecular and Cellular Biology, 4, Section 19-Section 19 (2000)
Eliza Zyłkiewicz et al.
The Journal of cell biology, 192(3), 433-445 (2011-02-02)
Ndel1 has been implicated in a variety of dynein-related processes, but its specific function is unclear. Here we describe an experimental approach to evaluate a role of Ndel1 in dynein-dependent microtubule self-organization using Ran-mediated asters in meiotic Xenopus egg extracts.

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