D2625

Dansyl chloride

BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)

• Synonym(s): 5-(Dimethylamino)naphthalene-1-sulfonyl chloride, DNSCl
• Empirical Formula (Hill Notation): C₁₂H₁₂ClNO₂S
• CAS Number: 605-65-2
• Molecular Weight: 269.75
• Beilstein: 2217205
• EC Number: 210-092-6
• MDL number: MFCD00003985
• UNSPSC Code: 12352101
• PubChem Substance ID: 24893700
• NACRES: NA.26

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: ≥99% (HPLC)
• form: powder and chunks
• color: yellow to yellow-orange
• mp: 72-74 °C (lit.)
• solubility: acetone: 20 mg/mL
• suitability: suitable for amino acid labeling
• storage temp.: 2-8°C
• SMILES string: CN(C)c1cccc2c(cccc12)S(Cl)(=O)=O
• InChI: 1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
• InChI key: XPDXVDYUQZHFPV-UHFFFAOYSA-N

Description

Application

A fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

Dansyl chloride has been used:

• as a fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase high performance liquid chromatography (HPLC)[1]
• in the derivatization of plasma samples[2][3]
• in the quantification of immobilized alkaline phosphatase[4]

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins.[5] Dansyl chloride is used for derivatization of free amino acids and amines[6] in neural samples and biological fluids.[7] It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.[7]

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H290,H314
• Precautionary Statements: P234 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves