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D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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5 ML
€234.00
5 X 5 ML
€896.00

€234.00

Please contact Customer Service for Availability
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5 ML
€234.00
5 X 5 ML
€896.00

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

€234.00

Please contact Customer Service for Availability

Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

Related Categories

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Components

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000411631
0000411631
0000375543
SLCN0620
0000320236

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Galanin receptor 3--a potential target for acute pancreatitis therapy.
Barreto SG
Neurogastroenterology and Motility, 23(3), 23(3)-23(3) (2011)
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
Gregor Obernosterer et al.
Nature protocols, 2(6), 1508-1514 (2007-06-16)
Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
M A Hull et al.
Gut, 43(4), 525-536 (1998-11-21)
Basic fibroblast growth factor (bFGF) promotes angiogenesis and healing of gastric ulcers in rats, and bFGF expression is up regulated in such ulcers. However, little is known about expression of bFGF in human gastric mucosa. To investigate bFGF expression in
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Protocols

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Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Questions

  1. Can you share the CAS # for the components (Ficoll and PVP) that are used to make the 50X Denhart's Solution?

    1 answer

    1. The CAS number for the Ficoll ingredient is 26873-85-8. The CAS number for the PVP ingredient is 9003-39-8 2.

      Below is the link to the Product Data Sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/172/670/d2532dat.pdf

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